ABSTRACT
The SARS-CoV-2 pandemic has elevated the development of novel diagnostic solutions, including rapid nucleic acid amplification tests (NAATs), to a global priority to meet the high demand for accurate, timely viral detection and diagnosis. However, ubiquitously implemented NAATs, such as polymerase chain reaction (PCR), consume hours of testing. We report a field-forward instrument capable of ultra-fast real-time PCR for amplification-based nucleic acid detection in a custom-designed microfluidic chip. Prudent selection and unconventional positioning of thermal cyclers relative to the microfluidic chip and a fluorescent detector permit ultra-fast simultaneous amplification and detection, with 40 cycles complete in under 10 minutes. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
ABSTRACT
Whether intertwined through wifi or plugged desktops, teenagers and young people are increasingly immersed in the ciberspace, due to the presence TDIC becoming ubiquitous. This study aims to comprehend how students from a public school in Bahia's countryside feel about having their lives more effectively in this space, as to relate socially, affectively, politically and so on in this pandemic context. It is an exploratory approach, with theoretical and methodological orientations that follow the New Studies of Literacies. The construction of the narratives happened through episodic interviews and the mining of the information over the Voyant Tools interface. From this interaction, it was possible to observe that some intermittences have provoked other sociocommunicatives becomings at the same time that some reverberations undertake that the participants of the research are in physical, but not social distancing, since sociability, too, happens over the virtual sphere.